DNA

Part:BBa_K1588006:Design

Designed by: Juan David Sifontes   Group: iGEM15_UNA_Honduras   (2015-09-18)


Protein with epitopes from dengue virus serotype 1 to generate immune response


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
    Illegal NgoMIV site found at 796
    Illegal NgoMIV site found at 1606
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 875


Design Notes

1. It is coded by just one gene 2. Is not too big 3. It is exported by bacterial cells 4. Has big hydrophylic loops to insert epitopes


Source

Vibrio cholerae VC_0620

References